Jackson was sent home to Detroit for tests, including an MRI, as well as examination by team doctors following Thursday's 2-1 loss to the Indians.
The Tigers had pushed back the decision by purchasing Quintin Berry's contract, but they had an unofficial deadline of Friday, when Ryan Raburn returns from the bereavement list for the series opener in Minnesota.
The extra time didn't help. Jackson didn't do any activity the past two days after feeling pain taking swings in batting practice on Tuesday night.
"It's somewhat getting better," Jackson said after Thursday's loss, "but it's still a little bit away from where it needs to be."
Raburn will take Jackson's place on the 25-man roster.
Teams can backdate a player's placement on the DL up to 10 days, and Thursday marked the eighth day since Jackson strained the abdominal muscle on a swing. He'll be eligible to return in time for the start of the next homestand, on June 1 against the Yankees.
Manager Jim Leyland does not like to discuss the impact of injuries, fearing it sends the wrong message to a team that has to overcome them. Still, he isn't downplaying this one.
"Everybody's making moves, everybody's got guys on the DL," Leyland said. "Jackson's [injury] is the one that hurts [us]."