Hollimon entered the game in the top of the eighth inning as a pinch-runner for Miguel Cabrera. He remained in the game at third base in the bottom of the inning and tried to make a diving play, when his left shoulder popped out upon impact.
An MRI exam will be conducted on Tuesday to get a better idea on the damage and how much time he'll miss.
Hollimon was expected to be headed to Minor League camp when the Tigers made their next round of cuts this week. The injury, however, puts his entire regular season at stake. He was expected to hold a middle infield job at Triple-A Toledo and serve as a utility option for the Tigers should they need an injury replacement at some point in the season.
The 25-year-old Hollimon ranked among the top prospects in the organization following the trades of the past offseason. He batted .282 with 34 doubles, 14 home runs and 76 RBIs in 127 games for Double-A Erie last season and played for Team USA last fall in the IBAF World Cup.
Jason Beck is a reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.